Chromatographic fingerprinting techniques plays an important and vital role in the process of standardization of herbal products. They are widely used for the confirmation of the presence of different herbs in the polyherbal formulations and the quantity of marker compounds. While HPLC and GC are widely utilized for chromatographic fingerprinting, HPTLC is the forerunner for chromatographic fingerprinting of polyherbal formulations and single log. Chromatographic fingerprinting is utilized for establishing the quality as well as the identity of single herbs, which are to be added in the herbal formulation. Nowadays, chromatographic fingerprinting is utilized indifferently in two different stages during the manufacturing of an herbal formulation. First fingerprinting is performed out for each and every herb that is being added to the herbal formulation. This process is used for recognizing the herb and can be used to simultaneously quantify the major active components present inside the herb. Once the purity and identity of the herb are established and recognized, all of the herbs are incorporated to produce the final polyherbal formulation.